Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcusxylosus Strain through Response Surface Modeling

Encouraging advances have been made in the application of microbial mineralization towards fixing and improving desertified sandy soils. However, desert soils arid areas exhibit high salinity that may limit urease activity production strains, thereby affecting solidification effects calcium binders saline In this study, a salt-tolerant strain (A80) produced was identified from Qaidam Basin. The culture conditions were optimized using single-factor tests response surface methods to optimize yields activity. optimal composition A80 medium included an inoculation amount 6.32% (V/V), yeast extract powder concentration 15.43 g/L, glucose 5.20 3%, incubation temperature 36 °C. Urease increased by 64.80% after medium. calcium-cementing agent also used solidify soils, leading unconfined compressive strength solidified soil 25.70%. Thus, optimization method resulted improvements cultivation strain.